Composite

Part:BBa_K1328002:Design

Designed by: Lingfeng Hou   Group: iGEM14_Tsinghua   (2014-10-09)


HXT1-CMVmini-EGFP-tADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 818
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 827
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part sequence contains EcoRI and PstI restriction sites, consider using SpeI and XbaI for cloning.


Source

Promoter region is assembled from parts: HXT1-CMVmini promoter (BBa_K1328001), EGFP (BBa_K1123017) and tADH1 transcription terminator.

References